20/07/2020 Offres d'emploi

Postodoral Position - Sensing and Profiling Extracellular Vesicles related to Neurodegenerative Diseases

Project : Sensing and Profiling Extracellular Vesicles related to Neurodegenerative Diseases Candidates should send a detailed CV with a letter of motivation and contact for reference people to both Souhir Boujday (souhir.boujday@sorbonne-universite.fr) and Christian Neri (christian.neri@inserm.fr).

Extracellular Vesicles (EVs) are key mediators of intercellular communication that are associated with several basic processes (e.g. development, maintenance) and human diseases (e.g. cancer, neurodegenerative disease). EVs have diagnostic potential as they may reflect the health/disease status of secreting cells and as they can be interrogated in biological fluids (e.g. blood). However, interrogating EVs in an easy, precise and reproducible manner is challenging due to their diversity (size, density, origin in the cell, cargo content) and to confounding factors (non-vesicular particles with similar physical features). Achieving this task is particularly important as specific EV subtypes may have specific functions.


In this project, they intend to design a direct, label-free biosensor for the precise detection and profiling of EV sub-types based on coupling quartz crystal microbalance with dissipation (QCM-D) and nanoplasmonic spectroscopy (NPS). They intend to combine both techniques for simultaneous real-time measurements to collect enriched information on biomolecular recognition under strictly identical conditions thanks to using a gold nanostructured plasmonic/QCM-D sensor chip coated with silica that is mounted in a QCM-D sapphire window module on top of which illumination in reflection mode is carried out by an optical fiber. The biosensor that will be developed herein will address concrete societal needs in precision medicine and health by rapid early diagnostic of neurodegenerative diseases and can be scaled to industrial level.


On the other hand, we have developed an expertise in engineering stable and reliable Bioconjugates combining antibodies and plasmonics nanoparticles. In this project we will synthetize and use different bioconjugates with defined shape and composition leading to identifiable responses in terms of optical properties (LSPR band and color) as well as different electronic properties allowing for their discrimination by Transition Electron Microscopy (TEM). This will be achieved starting from gold and/or silver nanoparticles used bare or coated by silica.


Candidates should be highly motivated with a strong background in colloidal synthesis and/or planar surfaces techniques related to biological applications.


The funding is for 1 year provided by the Institut des Sciences des Matériaux at the Sorbonne Université Alliance, starting in the fall 2020. Candidates should send a detailed CV with a letter of motivation and contact for reference people to both Souhir Boujday (souhir.boujday@sorbonne-universite.fr) and Christian Neri (christian.neri@inserm.fr).


REFERENCES

  • Souhir Boujday

doi.org/10.1021/acsami.9b14980
doi/10.1021/acs.jpcc.8b11864
10.1016/j.bios.2020.112370

 

  • Christian Neri

www.ncbi.nlm.nih.gov/pubmed/31113759
www.ncbi.nlm.nih.gov/pubmed/28178240
www.ncbi.nlm.nih.gov/pubmed/26681807